Composite

Part:BBa_K3610043:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-05)


EFR ectodomain / SmallBit for S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 355
    Illegal NheI site found at 1285
    Illegal NheI site found at 2203
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1871
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

HA tag fused to the luciferase for Western Blotting

Source

BBa_K319005, BBa_K2809002, BBa_K3610007, BBa_K416001, BBa_K3610016, BBa_K2753050

References

Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.

England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.